Biological activity of Tramesan on A. flavus and A. parasiticus.

In vitro culture of A. flavus and A. parasitucus under aflatoxin permissive conditions (PDB, 30°C) were treated or not (control) with 0.38 μM Tramesan and incubated for 7 days. Aflatoxin B1 production, evaluated by LC-MS/MS, and fungal growth, evaluated by weighting dried mycelia, in Tramesan-treated cultures, were normalised for non-treated ones and the percentage of inhibition calculated consequently. Mycelia was used to evaluate the expression, calculated by 2-ΔΔCt method in RT-PCR, of the oxidative stress related transcription factors AfyapA and ApyapA and the superoxide dismutase encoding gene sod1 in A. flavus and A. parasiticus, respectively.