Human intestinal organoid cell transcriptomic profiling. Human intestinal organoid cell transcriptomic profiling

We purified two populations of human ileal enteroendocrine cells (Venus+ and Venus-) from mature differentiated hGLU-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages. Overall design: Comparative transcriptomic study of 4x cell populations by bulk RNAseq, with 5x biological replicates in IF growth medium, and 3x biological replicates in IF* growth medium.