Plasmodium [Fe-S] biosynthesis pathway proteins of the apicoplast.

aGene IDs of the P. berghei and P. falciparum orthologs (http://PlasmoDB.org).bPutative targeting of the P. falciparum SUF pathway proteins to the apicoplast or mitochondrion was predicted using four different algorithms. ApicoAP [35] predicts whether a given protein lacks the required signal peptide (“No SP”), contains a signal peptide but no transit peptide (“non-ApicoTP”), or is an apicoplast targeted protein (“ApicoTP”) that uses the bipartite signaling mechanism. PlasmoAP [36] indicates the likelihood of the presence of the required signal peptide followed by the likelihood of an apicoplast localization (“-” = unlikely, “0″ = undecided, “+” = likely, “++” = very likely). PlasMit [37] predicts the likelihood of a mitochondrial localization for P. falciparum proteins (“non-mito (99%)”, “mito (91%)”, and “mito (99%)”). MitoProtII [38] gives a probability score for the likelihood of mitochondrial localization but is not optimized Plasmodium sequences. Note that no analysis was done for SUFB as the gene is encoded on the apicoplast genome and hence needs no targeting sequences.