mNET sequencing facilitates identification of native elongating transcripts in control and Carm1 KO melanoma cells

We investigated whether RNA Pol II mediated transcription was altered in Carm1 KO compared to control KO tumor cells. We examined p-Ser2 CTD Pol II relative to total CTD Pol II using mammalian native elongating transcript sequencing (mNET-Seq) (Nojima et al., 2016). Inactivation of the Carm1 gene substantially increased the normalized read density of p-Ser2 CTD Pol II tags relative total Pol II tags. These data provide evidence for altered transcriptional regulation in Carm1 deficient cells. Overall design: Examination of transcripts associated with Ser2 phosphorylated RNA Pol II in control and Carm1 KO melanoma cells.