Figure S1 - ALCAM Regulates Motility, Invasiveness, and Adherens Junction Formation in Uveal Melanoma Cells

Silencing of ALCAM in HEK cells results in disrupted ß-catenin junctions. HEK cells were transiently transfected with an shRNA construct confirmed to silence ALCAM (sh2), or a negative control scrambled shRNA (sh0). Both constructs included a GFP marker to track transfected cells (pseudocolored blue). HEK cells with silenced ALCAM expression show reduced ß-catenin localization (green) to cell-cell contacts, as well as reduced ALCAM expression (red; asterisk indicates an untransfected cell with a higher expression level of ALCAM). HEK cells transfected with the negative control shRNA, however, display robust ALCAM expression that localizes to cell junctions, and ß-catenin localizes strongly to cell junctions in these cells. (DOC)