Ten-hour time-restricted eating improves glycaemic control and alters transcriptomic profile in adipose tissue in men at increased risk of type 2 diabetes

Time restricted eating (TRE) is a simple intervention that has beneficial effects on glucose control in individuals with obesity, who are at high risk of metabolic diseases. TRE altered transcriptomic profile of adipose tissue but series sampling studies are need to evaluate the diurnal changes of gene expression. Overall design: In a single-arm, within-subject trial, 15 men (aged 62.9 ± 4 years, waist circumference 113 ± 4 cm) with no history of diabetes were enrolled and instructed to eat their regular diets within a contiguous 10-hour time frame each day for 8 weeks. The timing of food intake was tracked via a photograph-based application. After 2-weeks of baseline monitoring period and 8-weeks of TRE, participants were provided with a 3-day lead-in diet before they underwent a 35-hour metabolic ward stay, during which all food intake was strictly controlled within 14-hours (pre) or 10-hours (post). Identical standardized meal tolerance tests were performed at breakfast and dinner (52% carbohydrate, 33% fat, 15% protein). Transcriptional profiles in subcutaneous adipose tissue were measured in by RNA-sequencing (n=11).This study was designed to examine the effects of eight weeks of TRE (10-hours per day) on the glucose area under the curve (AUC) and appetite regulation at breakfast and dinner, and gene expression in adipose tissue in men with obesity.