Foci Detection and Colocalization 1052 Software.

Foci Detection and Colocalization Software. The Image and Data Analysis Core (IDAC) at Harvard Medical School developed a custom MATLAB based software for nuclear foci and colocalization detection in microscopy images based upon their previously described nuclear foci detection software. In brief, unaltered images are analyzed by the software to detect signal intensity over a set threshold to isolate foci from background. The software then uses DAPI staining to generate a mask that defines nuclear areas in the image. Only foci above the fluorescence threshold and within the nuclear masks are scored. The software determines colocalization based on the distance between the centers of nuclear foci in a reference channel to foci in other channels of the image.