Nitrobacter sp. CN101 genome sequencing

Nitrite is a key intermediate in global nitrogen cycles, but its accumulation is often not appreciable in environments. Conventionally, nitrite concentrations in media for cultivating nitrite-oxidizing bacteria (NOB) in laboratories may not be low enough to recover oligotrophic NOB. In this study, we newly designed a medium containing urea as a sole energy source for enriching oligotrophic NOB and obtained a pure culture designated as strain CN101. The complete genome of strain CN101 was compared with the genome of two previously sequenced Nitrobacter strains from soil. The genome of strain CN101 had more nitrite/nitrate transporters than that of the other Nitrobacter strains, which implies that the strain CN101 might flexibly adapt to varying nitrite concentrations in soils.