Transcriptome profiling of the response of Antarctic midge Parochlus steinenii larvae to heat and cold stress

Parochlus
steinenii is a species of winged midge found in the
Antarctic Peninsula. To understand the mechanisms they use to adapt to cold
environments and predict their responses to climate change, we investigate the transcriptome
of P. steinenii larvae under heat and
cold stresses. Here, we present the annotated genome sequence of P. steinenii with the transcriptome of
both adults and larvae, and differentially expressed genes due to cold and heat
stresses in larvae. A total of 12 757 genes were predicted, and gene set
completeness was 95.7 % in the BUSCO analysis against Eukaryota odb9. Of the predicted genes, 7 851 were
classified using Gene Ontology (GO), and genes differentially expressed more
than 2-fold were selected. In larvae, under cold stress, 75 genes were up-regulated
and 227 genes were down-regulated. In larvae, under heat stress at 12 °C and 20 °C, 37
genes were down-regulated, and three GO terms were statistically
overrepresented at both condition.