Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens

In the present study we investigate the role of the LysR-type transcriptional regulators LsrB (Atu2186) and OxyR (Atu4641) in the oxidative stress response of Agrobacterium tumefaciens C58 (alias A. fabrum C58). Therein, we used our existing lsrB-deletion (Kraus et al. 2020*) mutant and constructed two additional mutants: An oxyR and a lsrB/oxyR double deletion mutant. Total RNA was isolated from WT and mutant strains grown in LB medium to mid-exponential phase and withdrawn before or after 15 min, 5 mM H2O2 stress. Residual DNA was removed by RNAse-free Dnase. Sequencing was performed done on the Illumina NovaSeq 6000 platform. Our results reveal a central role for both LysR-type transcriptional regulators in the oxidative stress response of Agrobacterium tumefaciens. Kraus, A., Weskamp, M., Zierles, J., Balzer, M., Busch, R., Eisfeld, J., et al. (2020) Arginine-rich small proteins with a domain of unknown function DUF1127 play a role in phosphate and carbon metabolism of Agrobacterium tumefaciens. J Bacteriol 202: e00309-20. Illumina RNA sequencing of the transcriptomes of Agrobacterium fabrum C58 wild type; lsrB deletion mutant (∆atu2186); oxyR deletion mutant (∆atu4641); lsrB/oxyR double deletion mutant (∆atu2186∆atu4641) grown in LB medium +/- 5 mM H2O2