Mechanomics RT-DC Set 03 - HSPCs

Entry is a part of <b>Mechanomics</b> collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:<i>Urbanska, M., Ge, Y.</i> et al.,<br><b><i>De novo identification of universal cell mechanics gene signatures</i></b>BiorXiv: https://doi.org/10.1101/2021.04.26.441418<i>;</i><i> </i><i>first published:</i><i> </i>27th April 2021<i>, last updated:</i><i> </i>4th Nov 2024<br>eLife: https://doi.org/10.7554/eLife.87930<i>; first published:</i><i> </i>23rd March 2023<i>, last updated:</i><i> </i>27th Dec 2024<br>This dataset contains mechanical measurement of CD34-positive human hematopoietic stem and progenitor cells (HSPCs) isolated from mobilized peripheral blood and treated (Arulmozhivarman <i>et al.</i> 2017 <i>Sci Rep</i>):<br>i) with a histone deacetylase inhibitor, valproic acid (VPA)<br>ii) untreated control (PBS).<br><b>Of note: </b>This dataset was removed from later versions of the manuscript. Measurements were performed using real-time deformability cytometry (RT-DC).<br><b>_rtdc.zip </b>- contains data acquired during the measurments in '*.rtdc' format<b>_tsv.zip </b>- contains extended data in a '*.tsv' format<br><b>_glmm.zip </b>- contains result summaries of statistical analysis comparing Young's moduli of the different cell types using generalised linear mixed effects models<br>Arulmozhivarman, G. <i>et al.</i> Zebrafish In-Vivo Screening for Compounds Amplifying Hematopoietic Stem and Progenitor Cells: Preclinical Validation in Human CD34+ Stem and Progenitor Cells. <i>Sci Rep</i> 7, <b>12084</b> (2017). https://doi.org/10.1038/s41598-017-12360-0