DNA repair in oocytes

Sequencing of offspring tails from TP63-/- female mice irradiated with 45Gy and non irradiated controls to determine mutation rate at a dose that sterilises wild type mice.Despite mobilizing the DNA repair response, even very low levels of DNA damage in wild type mice results in the apoptosis of prophase-arrested oocytes. This study is to determine if when apoptosis is inhibited, severe DNA damage is corrected via homologous recombination repair and if repair is sufficient to support genetic fidelity in offspring.