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The study was conducted as an experimental interventional study in which each animal served as its control. The experiment included a 3 day control period before OF overload (0 h) and 3 days of surveillance afterward. All animals received the same experimental treatment. According to a previous study (7), the OF-treated heifers (n = 5) received 17 g/kg body weight (BW) of OF (Bailong Bio-Tech Company, Jinan, China) dissolved in 2 L of tap water per 100 kg of BW. Before 3 days (–72 h) of the high oral dose of OF, a 5% dose of OF was administered daily. During the preexperimental period, heifers were trained to have their front feet lifted and examined, and to be directed by hand to walk and trot. During the experiment, all heifers were housed in a tie-stall barn on a rubber-layered floor bed and fed grass hay ad libitum. After 72 h of OF overload, heifers were euthanized.