S1 File - Elucidating the Influence of Gold Nanoparticles on the Binding of Salvianolic Acid B and Rosmarinic Acid to Bovine Serum Albumin

Figure A, Fluorescence titration curves of BSA in the presence of SAB/RA at λex = 280 and 295 nm. CBSA = 5.0 × 10-6 mol L-1, T = 298 K, pH = 7.4. (A): BSA-SAB system, (B): BSA-RA system, (C): BSA-SAB-Au4 system, (D): BSA-RA-Au4 system, (E): BSA-SAB-Au3 system, (F): BSA-RA-Au3 system, (G): BSA-SAB-Au2 system, (H): BSA-RA-Au2 system. Figure B, Overlapping of the fluorescence emission spectrum of BSA (1) with the absorption spectrum of drug (2). (A): BSA-SAB system, (B): BSA-RA system, (C): BSA-SAB-Au4 system, (D): BSA-RA-Au4 system, (E): BSA-SAB-Au3 system, (F): BSA-RA-Au3 system, (G): BSA-SAB-Au2 system, (H): BSA-RA-Au2 system. The concentrations of drug and BSA were 5.0 × 10-6 mol L-1 and the concentration of Au NPs were 10.0 × 10-11 mol L-1, λex = 280 nm, T = 298 K. Figure C, The three-dimensional fluorescence spectra (A, B, C, D, E, F, G, H, I, J, K, L) and corresponding contour spectra (A’, B’, C’, D’, E’, F’, G’, H’, I’, J’, K’, L’) of drug-BSA systems. (A, A’): BSA system, (B, B’): BSA-SAB system, (C, C’): BSA-RA system, (D, D’): BSA-Au4 system, (E, E’): BSA-SAB-Au4 system, (F, F’): BSA-RA-Au4 system, (G, G’): BSA-Au3 system, (H, H’): BSA-SAB-Au3 system, (I, I’): BSA-RA-Au3 system, (J, J’): BSA-Au2 system, (K, K’): BSA-SAB-Au2 system, (L, L’): BSA-RA-Au2 system. CBSA = 5.0 × 10-6 mol L-1, CSAB = CRA = 10.0 × 10-6 mol L-1, and the concentration of Au NPs were 10.0 × 10-11 mol L-1. (DOCX)