Cell lines used to study MCVSyn replication, early and late transcription as well as particle formation.
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a: LT-Ag protein expression was tested by Immunoblotting 2d, 4d, 8d, 12d, 44d past transfection;b: transcripts were tested at 2d, 4d, 8d and 12d p.t. using quantitative realtime PCR and normalized to GAPDH. n.t.: not tested.c: Transfection efficiency was determined for each cell line using 100 ng GFP expression construct in 105 cells, 48 h p.t cells were analysed by FACS for GFP expression.
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2015-12-02



