Raw HPLC-MS/MS data for "Oxidative stress is inhibited by plant-based supplements: a quantitative lipidomic analysis of antioxidant activity and lipid compositional change"

Data are the raw data for our targeted lipidomics study testing the antioxidant properties of several dietary supplements derived from plant extracts against a complex lipid mixture. Five supplements were tested for antioxidant activity these contained grape seed extract, hawthorn extract, pine bark extract, milk thistle extract, and turmeric extract. Overall the data show that most of the supplements had an antioxidant effect on the phosphatidylethanolamine lipid class but grape seed extract was effective across most of the 15 lipid classes and circa 400 hundred lipid species identified. Experiments were performed using a liposomal oxidation assay with the radical peroxidation agent 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH) in the presence and absence of supplement at 0, 6 and 24 hrs post oxidation initiation. Lipidomic data were obtained using high pressure liquid chromatography mass spectrometry (HPLC-MS). Reanalysis is possible using a number of metabolomic or lipidomic platforms and these data may prove useful as the field of oxidative lipidomics develops, since we were unable to reliably identify a significant number of chromatographic features.