Diversity patterns of uncultured Haptophytes unravelled by pyrosequencing in Naples Bay
收藏DataONE2022-09-20 更新2025-09-20 收录
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Haptophytes are a key phylum of marine protists, including ~300
described morphospecies and 80 morphogenera. We used 454-pyrosequencing on large
subunit ribosomal DNA (LSU rDNA) fragments to assess the diversity from size
fractioned plankton samples collected in the Bay of Naples. One group-specific
primer set targeting an overlapping region of the LSU rDNA D1/D2 region was used to
amplify Haptophyte sequences from nucleic acid extracts (total DNA or RNA) of two
size fractions (0.8-3 or 3-20 µm) and two sampling depths (subsurface, at 1m, or
deep-chlorophyll-maximum (DCM) at 23m).
创建时间：
2025-09-16
