Gene disruption by CRISPR/Cas9 is highly efficient and relies on the error-prone non-homologous end-joining (NHEJ) pathway. Conversely, precise gene editing requires homology-directed repair (HDR), wh
Genetic improvements and RNA-guided genome editing of plants use clustered regularly interspaced short palindromic repeats (CRISPR) associated with a potential nuclear-localized endonuclease (Cas9). D
By applying a barcoding strategy to clonal tracking of edited cells (BAR-seq), we show that p53 activation triggered by HDR editing significantly shrink the HSPC clonal repertoire in hematochimeric mi