RAD-seq reduced-genome sequencing of 262 S. cerevisiae strains for phylogenetic analysis

We applied RAD-seq, a multiplexed, reduced genome sequencing strategy, to genotype a large collection of S. cerevisiae strains, isolated from a wide range of geographical locations and environmental niches. The method permits the sequencing of the same 1% of all genomes, producing a multiple sequence alignment of 116,880 bases across 262 strains