Native <i>T</i>s-Pmy from <i>T</i>. <i>spiralis</i> worm bound to human C1q.

Protein G Micro Beads were pre-incubated with anti-Ts-Pmy mAb 9G3 and T. spiralis adult worm crude extracts. Then, human complement C1q was added and the incubation was continued for 2h. The bound proteins were subjected to SDS-PAGE and transferred to a NC membrane. The membrane was probed with an anti-C1q mAb. The consistent results are repeated for three times. M, standard protein marker; Lane 1, pulled down immune complex (worm extracts + anti-Ts-Pmy + C1q); Lane 2, human C1q alone (2 ug); Lane 3, human C1q with the anti-Ts-Pmy mAb 9G3 alone without the addition of T. spiralis adult extracts.