RNA-seq analysis of WT, abs3-1D, two abs3-1D suppressor lines (abs3-1D arf2-20 and abs3-1D pif5-10), and suppressor single mutants (arf2-20 and pif5-10) before and after 4 d C-deprivation treatment.
A suppressor of the short-hypocotyl phenotype of shv3svl1 was mapped to suc1, encoding a plasma membrane Suc/H+ symporter. The EMS-induced suppressor mutant was backcrossed to the shv3svl1 progenitor
JE2 was passaged for 5 days in 96 well plates containing selective media, Grown cells were re-passaged for the duration of the passaging experiments. combination treated cells were prioritised for WGS