List of compounds used to validate the assay.

Their host cell toxicity (TC50) and anti-T. cruzi (IC50 for the ‘Am/Cell’ and ‘%Infectivity’ assay outputs) mean values of three independent experiments plus their sd are shown (mean ± sd). aIC50 values for the ‘Am/Cell’ output; bIC50 values for the ‘%Infected’ output; cratio between the mean TC50 values and their corresponding ‘Am/Cell’ IC50 measurement. dliterature reference; end, not determined; #values obtained at the anti-Chagas HTS campaign recently performed at GSK (Peña et al., manuscript in preparation); *not actually an IC50 value but the minimal compound dose to eradicate T. cruzi amastigotes in Vero cells [26]. List of compounds used to validate the assay.