Transcriptome-wide maps of m6A RNA methylation marks in murine liver

N6-methyladenosine (m6A), the most prevalent modification of mRNAs at the (G/A)(m6A)C motif installed by METTL3 that plays critical role in regulation of gene expression. METTL3 is essential for embryonic development, and its dysregulation is linked to various diseases including metabolic disorders and cancer. Here, we provide insight into this by generating Mettl3fl/fl; Alb-Cre (M3LKO) mice carrying a hepatocyte-specific deletion of Mettl3. RNA-seq and MeRIP-seq analyses of mRNA identified significant dysregulation of metabolic genes in M3LKO mice. Overall design: Enrichment of m6A methylated RNA in 4 murine WT and METTL3 knockout livers, and transcriptome-wide sequencing of polyadenylated RNA in 6 and 8 WT and METTL3 KO livers, respectively.