Genome-wide analysis of pluripotency marker expression of ESKM-iPS cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE28594
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iPS cells, produced in in presence of exogeneously expressed E-cadherin and abcence of viral Oct4 were compared with conventional produced OSKM-iPS cells and murine embryonic stem cells (mESCs) or MEFs Total RNA was isolated from iPSCs and mESCs grown in presence of LIF on gelatin-coated plates for 4 days and from murine embryonic fibroblasts grown in standard medium (DMEM-Glutamax, high-glucose, 10 % FBS, 1X non-essential amino acids, penicillin/ streptomycin
创建时间:
2012-08-14



