A rat insulinoma cell line (INS-1) was generated that contains a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the line contains the tetracycline repr
The purpose of the experiment was to investigate the heterogeneity of islet cell response, especially beta-cells to hyperglycemia induced by severe insulin resistance in mice. Overall design: Mice w
Ins-1E cells were incubated for 1 h at 37 ºC with unlabelled apoA-I (0.9 mg/mL) and Alexa Fluor 488-labelled apoA-I (0.1 mg/mL) under basal glucose conditions. The cells were harvested and incubated
