Table_3_Non-targeted metabolomics reveals the taste variations during Baccaurea ramiflora Lour. fruit maturation.xlsx

Baccaurea ramiflora Lour. is a new kind of underutilized wild fruit tree; the metabolic reasons for its fruit flavor changes are not yet clear. In this study, the pink flesh of this excellent tasting fruit (BR) was used to reveal the metabolic causes of taste variations through five developmental stages. We identified 154 common differential metabolites of different developmental stages based on non-targeted metabolomics analysis. The accumulation of sugar and fatty acids increased significantly after 73 days, while citric acid decreased significantly. Flesh color accumulation mainly occurred 53 days ago, and vitamin accumulation occurred after 93 days. Interestingly, L-sorbose and 5-hydroxyindole-3-acetic acid were positively correlated with the sugar–acid ratio but negatively correlated with titratable acids. It indicated that L-sorbose and 5-hydroxyindole-3-acetic acid may be taste biomarkers of BR B. ramiflora. The results provided new metabolic lines of evidence for the taste variation during the ripening process of B. ramiflora.

Baccaurea ramiflora Lour. 为一种新型未被充分利用的野生果树；其果实风味变化的代谢原因尚不明确。本研究中，以该优异口感果实的粉红色果肉（BR）为研究对象，通过五个发育阶段，揭示了风味变异的代谢成因。基于非靶向代谢组学分析，我们鉴定了154种不同发育阶段的常见差异代谢物。糖和脂肪酸在73天后显著积累，而柠檬酸则显著降低。果肉色泽的积累主要发生在53天前，而维生素的积累则在93天后出现。有趣的是，L-苏糖和5-羟基吲哚-3-乙酸与糖酸比呈正相关，但与可滴定酸呈负相关。这表明L-苏糖和5-羟基吲哚-3-乙酸可能是BR B. ramiflora的风味生物标志物。研究结果为B. ramiflora成熟过程中风味变异提供了新的代谢学证据。