UUO-rUUO small RNA seq, Denby lab 2019

Small RNA sequencing of sorted cell type, mice kidneys, after unilateral ureteral obstructive (UUO) surgery after 2 days, 7 days and a reversible (rUUO) 14 day model with corresponding sham operated mice. The goal of the study is to identify micro-RNA dysregulation of expression between different cell types corresponding with kidney injury, in order to identify and assess biomarkers which could be translated into the clinical setting. Overall design: Each group was sequenced from 4 mice, and 4 cell types. 2 day UUO kidneys were sorted into PDGFRß+, F480+, LTL+ and CD31+ cell types before the RNA was extracted and small RNA sequenced for 4 mice. The same protocol was followed for 7 day UUO mice, rUUO mice, as well as sham operated mice for reference.