Expression data from human endometrial stromal cells (HESCs) treated with PRP

In IVF treatment, the effects of intrauterine platelet-rich plasma (PRP) infusion with increasing rate of successful pregnancy have been reported; however, the mechanisms that support embryo implantation remain unclear. In the undifferentiated HESCs, PRP was found to strongly promote the gene expression associated with cell growth, tissue regeneration, proinflammatory response, and antibiotic effects. In decidualized HESCs, PRP was found to attenuate the gene expression involved in cell proliferation and inflammation by inhibiting the expression of PI3K signaling. HESCs were cultured and passaged equally into four cell culture dishes in each patient. Two were treated with PRP twice and the other two were treated with vehicle. Subsequently, two cultures with and without PRP were decidualized with cAMP and progesterone for 5 days. The gene expression in undifferentiated or decidualized HESCs with and without PRP was compared.