File S1 - Detergent Screening and Purification of the Human Liver ABC Transporters BSEP (ABCB11) and MDR3 (ABCB4) Expressed in the Yeast Pichia pastoris

Combined file of supporting figures and tables. Figure S1: Dot Blot quantification of BSEP (A), BSEP-GFP (B) and MDR3 (C). Average values from two independent dot blots are shown (n = 2) ± SD. Large errors for e.g. the Fos-Choline series resulted from saturation of the detector. The intensity of SDS was set to 100% and all other values were normalized to SDS. Black bars represent zwitter-ionic detergents, grey bars ionic detergents and white bars non-ionic detergents. Figure S2: FSEC profiles of free GFP and MDR3-GFP in selected detergents. The x-axis shows time in minutes, the y-axis fluorescence in arbitrary units. Figure S3: FSEC profiles of BSEP-GFP in selected detergents. The x-axis shows time in minutes, the y-axis fluorescence in arbitrary units. Table S1: Used detergents for solubilization of BSEP and MDR3 and Dot Blot analysis; N: Non-ionic detergents; Z: Zwitterionic detergents; A: Anionic detergents; C: Cationic detergents. (DOCX)