PTP1B deficiency effect on mammary gland development and differentiation

Analysis of protein tyrosine phosphatase 1B (PTP1B) deficient mammary glands from nulliparous mice at estrous and pregnancy day 3, 7, 10 and 15. We used a genetically ablated PTP1B mouse model to gain a deeper knowledge of the role PTP1B plays in mammary gland development and to define the mechanism regulated by this phosphatase. Results provide insight into the role of PTP1B in mammary gland development and differentiation. Mouse mammary glands were isolated from PTP1B -/- and PTP1B +/+ nulliparous mice at estrous and pregnancy day 3, 7, 10 and 15 for RNA extraction and hybridization on Affymetrix microarrays.本研究针对动情周期及妊娠第3、7、10、15天的未产雌鼠体内的蛋白质酪氨酸磷酸酶1B（protein tyrosine phosphatase 1B，PTP1B）缺陷乳腺组织展开分析。本研究采用基因敲除PTP1B小鼠模型，以期深入解析PTP1B在乳腺发育中的作用，并明确该磷酸酶所调控的分子机制。研究结果可为阐明PTP1B在乳腺发育与分化中的功能提供新的见解。研究人员从动情周期及妊娠第3、7、10、15天的PTP1B基因敲除纯合子（PTP1B-/-）与野生型纯合子（PTP1B+/+）未产雌鼠体内分离乳腺组织，用于RNA提取及Affymetrix基因芯片杂交实验。