Whole genome transcription profile of activated pre-B cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE38796
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To search for rapid changes in gene expression following pre-BCR activation, we performed DNA microarray analysis of activated pre-B cells with and without anti-m heavy chain treatment for 3 hour.The expression of only small subset of genes differed significantly. The mouse bone marrow derived pre-B cells were grown with IL-7 on limiting amounts of S17 stromal cells and activated by incubation with goat F(ab’)2 anti-mouse μ heavy chain (Southern Biotechnologies) at 5 μg/ml for 3 hour.
创建时间:
2019-01-16



