sub-PEN-seq for identifying novel non-coding RNAs and their expression in cultured cells II
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https://www.ncbi.nlm.nih.gov/sra/SRP334234
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资源简介：
Small RNAs between 20-500nt were least explored. Here we used RNase H-based rRNA depletion and a serial of strategies to developed sub-PEN-seq method for identifying novel non-coding RNAs in Subcellular RNAs. Overall design: Subcellular RNA were ligated to RNA adaptors, followed by rRNA and snRNA depletion. After reverse transcription and library amplification, inserts between 20-500bp were size selected and subjected to Illumina sequencing.
创建时间：
2023-05-10
