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CIL: 50915, Caenorhabditis elegans (roundworm, nematode), Intestine cells and Oocytes. In Cell Image Library

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DataCite Commons2025-10-31 更新2026-05-06 收录
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https://library.ucsd.edu/dc/object/bb3422584v
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Worms were cultured onto normal growing medium plates spread with Escherichia coli (OP50 strain) and high pressure frozen in a Wohlwend Compact 3 machine, followed by a short freeze substitution protocol in 2% Uranyl acetate and embedded in HM20 resin. Sections of 70 nm thickness were then contrast stained with 2% Uranyl acetate and Reynold's lead citrate. Pictures were aquired in a Tecnai T12 TEM with a Ceta CMOS 16M camera.
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UC San Diego Library Digital Collections
创建时间:
2021-04-16
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