Lack of Wobble Uridine tRNA Modification Affects Translation Efficiency.

aThe strains were grown at 20°C before heat-shock at 33°C. The difference of β-gal activity (mean±SD) between N2 and elp-1(tm2149), elp-3(tm3120) or tuc-1(tm1297) was analyzed by student's t test (*p<0.01). For the QRT-PCR data (mean±SD), the lacZ mRNA was normalized against tbb-2 and ubc-2 mRNA (data not shown). In the table are the ratios between lacZ and tbb-2 mRNA. There is no reduction on mRNA level in the mutant strains.
bThe strains were grown at 15°C before heat-shock at 33°C. The reduction of β-gal activity (mean±SD) in elp-1(tm2149); tuc-1(tm1297) is significant compared to the wild type (**p<0.001). The lacZ mRNA level (mean±SD) was also normalized against tbb-2 and ubc-2 mRNA (data not shown).