Transriptional profile of various BDNF forms increased expression in rat neonatal mPFC.

In this work we studied influence of temporal expression elevation of pro- , mature and mutated BDNF form that could not be processed with Furin in neonatal medial prefrontal cortex (mPFC) on anxiety and depressive like behavior in juvenile age (P30). With lentiviral doxycycline inducible constructs we expressed various BDNF forms in male neonatal rat pups mPFC from P3 to P8. Lentiviruses were stereotacticaly infused at P1. Using RNA-sequencing, we studied transcriptomics changes in neonatal mPFC caused by expression of different BDNF forms. Temporal expression of them in neonatal mPFC had distinct transcriptional signatures at P8 and affected anxiety and depressive like behavior in juvenile rats.