Gene regulation by TLR2 and TLR10 in oncogene-induced senescence

In the present study, we analyse the effect of knocking down TLR2 and TLR10 during oncogene induced senescence in IMR90 cells expressing a inducible version of oncogenic RAS (ER:RAS) in the transcriptome using Ampliseq RNA sequencig. We observed that TLR2 and TLR10 regulate the expression of many pro-inflammatory cytokines and chemokines that constitute the senescence associated secretory phenotype. There is also significant regulation of genes of the acute phase response. Overall design: IMR90 expressing ER:RAS fusion protein induced to senescence with activated oncogenic RAS by addition of 4 hydroxy-tamoxifen (4OHT), were transfected with siRNA to knockdown of TLR2 and TLR10. Total mRNA was extracted and expression profiles were analysed.