Flow cytometry data – BE134: dual-parameter replicative age and viability profiling of Saccharomyces cerevisiae var. diastaticus

BE134 — Saccharomyces cerevisiae var. diastaticus Strain: SafAle™ BE-134 (Saccharomyces cerevisiae var. diastaticus), industrial ale yeast. Role: application dataset. Data: 33 FCS files. Measurements performed exclusively in ICP buffer at pH 3, 4, 5, and 6 (no MOPS buffer), with three biological replicates per condition. Three measurement conditions: mCherry fluorescence without dye (Age_I, 12 files, pH 3–6), mCherry fluorescence with CFDA staining (Age_I_CFDA, 9 files, pH 3–5), and unstained reference controls (RI, 12 files, pH 3–6). Analysis: Analysis workflow identical to W34/70 and X33, with an adapted viability threshold: scale group B (FITC-A/PerCP-A threshold 10.0), reflecting the substantially higher baseline fluorescence of this strain. Data at pH 3, 4, and 5 were used for analysis; pH 6 files are present in the dataset but were excluded from analysis to maintain comparability with LA01. No MOPS buffer overrides required.