Reconstitution upon third WBMCs transplantation.
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Third transplantation: 2×105 CD45.2 cells sorted from 2nd recipient WT or CL mice, plus 2×105 CD45.1 WBMCs were transplanted into lethally irradiated (9.5 Gy) recipients (B6. SJL, CD45.1). Flow cytometry analysis of PB was performed after 8 weeks of transplantation. If the number of engrafted CD45.2+ cells in PB is greater than 1%, it was considered as positive reconstitution. Data were analyzed by χ2, P
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2015-12-02



