PARP7 is a monoPARP that catalyzes the transfer of single units of ADP-ribose onto substrates to change their function. PARP7 expression is increased by aromatic hydrocarbons and cellular stress, and
CRISPR screen: U2OS or U2OS p53KO cells expressing Cas9 were transduced with a whole-genome library of CRISPR sgRNAs, then treated with either DMSO or etoposide. Differential sgRNA abundances were cal
Purpose: The aim of this experiment was to determine the effect of SNAT1 loss on Th1 and Th17 cells. Methods: SNAT1 was knocked out using CRISPR/Cas9 in primary CD4+ T cells polarized to Th1 and Th17