Single-Cell Transcriptomic Profiling of colonic wounds and intact tissues in Hgfac WT and KO mice

Wound healing is orchestrated by a spatial and temporal network of intercellular communication between epithelial cells, the stromal compartment, and the immune system. We found that Hgfac KO mice showed delayed wound healing in an endoscopic wound model. To dissect the celluar and molecular mechanisms of tissue healing, we collected tissues from day2 wounds or intact tissues from Hgfac WT and KO mice using a skin biopsy punch and dissociated cells for scRNA-seq analysis. To identify transcriptional programs associated with wound healing in various cell types and to analyze whether there is a difference between Hgfac WT and KO mice.