Paul S. Maddox, Francie Hyndman, Joost Monen, Karen Oegema, Arshad Desai (2011) CIL:28783, Caenorhabditis elegans, embryo. CIL. Dataset

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 (strain OD31) in which KNL-1 has been depleted by RNAi. Discrete foci of GFP signal within the nucleus coalesce along curvilinear chromosomes during prophase. After nuclear envelope breakdown, chromosomes fail to congress to the metaphase plate, but GFP-KNL-2 localization is evident. As seen after CeCENP-A depletion and CeCENP-C depletion (CIL 28779 and CIL 28782, respectively), foci of GFP-KNL-2 formed along the spindle after anaphase onset. The video is ∼11 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals (see Materials and methods), and corresponding data are shown in Fig. 3 in J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5.

对表达GFP-KNL-2（菌株OD31）并经RNAi处理以耗竭KNL-1的秀丽隐杆线虫胚胎中第一次有丝分裂进行旋转盘共聚焦时间间隔成像。在前期，核内的GFP信号离散焦点沿曲线染色体融合。核膜破裂后，染色体未能向中期板聚集，但GFP-KNL-2的定位清晰可见。如同CeCENP-A耗竭和CeCENP-C耗竭后所见（分别见CIL 28779和CIL 28782），GFP-KNL-2的焦点在后期开始后沿纺锤体形成。视频时长约为11分钟，帧高约30微米。图像以10秒为间隔采集（详见材料和方法的描述），相关数据展示于《细胞生物学杂志》2007年3月12日第176卷第6期第757-763页，电子版发布于2007年3月5日。