Lemonade Creek, Yellowstone National Park, USA - Microbial Community Analysis - Genome and Transcriptome Data

Genome and Transcriptome data used for analysis of microbial community function over a diurnal cycle in Lemonade Creek, Yellowstone National Park, USA.

mags.tar  Non-redundant metagenome data (genome assemblies, predicted genes, and gene functional annotations).

In each directory are the the following files:

- *.mRNA.faa protein sequences of protein-coding genes

- *.mRNA.fna nucleotide sequences of protein-coding genes

- *.mRNA.gff3 genomic location of protein-coding genes

- *.mRNA.emapper.tsv eggNOG-mapper annotations for the protein-coding genes

- *.mRNA.interproscan.gff3 InterProScan annotations for the protein-coding genes

In the prokaryote directory there are the following files:

- *.rRNA.fna nucleotide sequences of rRNA genes

- *.rRNA.gff3 genomic location of rRNA genes

- *.tRNA.fna nucleotide sequences of tRNA genes

- *.tRNA.gff3 genomic location of tRNA genes

- *.other.fna nucleotide sequences of other genes (i.e., CRISPR, ncRNA, oriC, regulatory_region, repeat_region, tmRNA - if any were predicted)

- *.other.gff3 genomic location of other genes

Eukaryotes

Five MAGs from other eukaryotes that were assembled from a coassembly of the Soil samples.

Prokaryotes

The final dereplicated prokaryote MAGs (at 95% ID). The two *stats* files list the taxonomic information (from GTDB-Tk), completeness (from CheckM), and assembly stats (from the stats.sh script from the bbmap package) for each of the prokaryotic MAGs + the number of predicted protein-coding and non-protein-coding genes predicted in each MAG.

Viruses

The final dereplicated viral MAGs and vOTUs.

read_mapping.tar Abundance results from metagenome and metatranscriptome read mapping analysis against the non-redundant metagenome data and predicted genes (respectively). This analysis includes the cyanidiophyceae reference nuclear and organelle genomes.

mags

Results from bbmaps alignment of metagenome reads against a database of non-redudant metagenome MAGs + cyanidiophyceae reference nuclear and organelle genomes. CoverM was used to calculate MAG abundances.

genes

Salmon abundance quantification of PolyA and RiboMinus metatranscriptome reads mapped against the predicted genes in the non-redudant metagenome MAGs + cyanidiophyceae reference nuclear and organelle genomes.