Cytosine methylation dynamics during spermatogenesis and post-testicular sperm maturation. Mus musculus

We carried out whole genome bisulfite sequencing in seven purified sperm populations, including primary spermatocytes, early and late round spermatids, and mature sperm isolated from the caput, corpus, and cauda epididiymis, and from the vas deferens. Overall design: Testicular sperm populations were isolated by elutriation. Epididymal and vas deferens sperm were obtained by gently squeezing sperm out of the dissected tissue. Sperm were washed several times prior to genomic DNA extraction, and purified gDNA was subject to WGBS.