Individually tested InDels.

Noted within the sequence column, in bold, is the inserted base, and in bold with strikethrough the deleted one.aThe TRD recognition residues interact with the substrate DNA and are located in amino acids 219 to 244 [18], [50].bMTase activity relates to the fraction of plasmid DNA that was completely methylated and hence protected from digestion by HaeIII; the fraction was deduced from the gel image (Figure 3) using GelAnalyzer.cThe conservation score derived from Consurf [49] using the multiple sequence alignment of M.HaeIII's orthologs with standard parameters (see Figure 1D).