Flow cytometry fluorescence time course of S. enterica harboring plasmid Ppdu–GFP.

Time is indicated as hours after OD600 = 0.4, at which point cultures continued to grow without 1,2-PD (open symbols) or with the addition of 1,2-PD (solid symbols). (A) Wild-type S. enterica grown in NCE minimal media. (B) Wild-type S. enterica grown in LB media. (C) Wild-type S. enterica grown in LB carrying a secondary plasmid containing either the control vector pTET MBP without aTc (squares), or pTET pocR induced with 1 ng/mL aTc (triangles). (D) S. enterica ΔpocR grown in LB carrying a secondary plasmid containing either the control vector pTET MBP without aTc (squares), or pTET pocR induced with 1 ng/mL aTc (triangles).