the raw data for manuscript 1118778.xlsx

Electroacupuncture (EA) therapy, as a combination of electric stimulation and traditional acupuncture technology, is currently an important mean for treatment of ischemic stroke and post-stroke rehabilitation in China. Because of its remarkable therapeutic effect, it has been widely used in hospitals and clinic, however, the detailed mechanism remains unclear. Previous experimental studies, due to technical limitations, could only focus on a single pathway or mechanism for analysis, and could not comprehensively and systematically summarize mechanisms of EA on treatment of ischemic stroke. In this study, we used middle cerebral artery occlusion (MCAO) in rats to simulate process of cerebral ischemia-reperfusion (I/R), and used RNA-Sequencing (RNA-Seq) to detect transcriptomic changes of various molecules in ischemic hippocampal neurons of rats in Sham, I/R and EA groups. Thus, we detected 18 significantly different genes related to atherosclerosis (AS), including lipid metabolism, thrombosis, monocytes and vascular smooth muscle cells. And, we detected 10 significantly different genes related to oxidative stress and apoptosis and 10 significantly different genes related to calcium overload and excitatory amino acids release. As well as, we detected 19 significantly different genes related to blood-brain barrier (BBB) and 22 significantly different genes related to inflammatory response. In conclusion, EA can play a role in treating ischemic stroke through a variety of mechanisms, including atherosclerosis, oxidative stress, apoptosis, calcium overload, excitatory amino acids release, blood-brain barrier (BBB) and inflammatory response.