Additional file 3: of IRES-dependent translated genes in fungi: computational prediction, phylogenetic conservation and functional association

Comparison of ribosome profiles. Ribosome profiles for Saccharomyces cerevisiae grown in rich media and under starvation conditions are presented. A) and B) are two genes that have verified IRESs. C) and D) are positive predictions and E), and F) are negative predictions. The regions presented comprise the 60 nt upstream and the 100 nt downstream the start codon. The translation initiation site is shown (blue vertical lines). The genes having IRESs present an increment ribosomes occupancy within the 60 nt upstream the translation initiation in starvation conditions compared to rich media. The two positive predictions depicted similar increments. These increments are not observed in the two negative predictions. The profiles were obtained using the web page http://gwips.ucc.ie/ . (PNG 980 kb)