Transcriptional Profiling of Dorsal Root Ganglion Neurons Following Sciatic Nerve Injury. Transcriptional Profiling of Dorsal Root Ganglion Neurons Following Sciatic Nerve Injury

To elucidate transcriptional responses in peripherally injured neurons, we performed RNA-seq of dorsal root gangliona (DRG) sensory neurons at 1 and 5 days following sciatic nerve injury. Overall design: Sciatic nerve crush was performed by exposing the left sciatic nerve at the level of sciatic notch and crushing with smooth forceps for 30s. L3-6 DRGs from naïve or crushed mice were dissected and vGLUT2-tdTomato labeled neurons were dissociated and selected by fluorescence-activated cell sorting (FACS), then mRNA purified and profiled by sequencing.