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Development and Clinical Evaluation of a Next-Generation Sequencing Approach for HIV-1 Genotypic Resistance Testing

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP271001
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HIV-1 Genotypic Resistance Testing on clinical specimens using Next-Generation Sequencing in the Miseq platform. Fragment libraries were prepared from PR/RT and IN amplicons produced by one step RT-PCR followed by nested PCR.Goal: To compare between Sanger and Miseq-NGS consensus sequences, predicted levels of drug resistance, and the efficiency of detecting low-level mutations in clinical samples.
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2020-07-09
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