Analyzing the Impact of RNA Lipid Aggregates on Fibroblast Reticular Cells via RNA Sequencing In Vitro

Fibroblastic reticular cells (FRCs) were cultured in 6-well plates and treated with lipid particles (NP samples, n = 3 wells) or RNA-loaded lipid particle aggregates (RNA samples, n = 3 wells). 24 hours later, the cells were harvested, and RNA immediately extracted. The RNA samples were then sent to Novogene for library preparation. Sequencing was conducted on the Illumina NovaSeq 6000 platform, utilizing a paired-end 150 bp sequencing approach. The RNA used for treatment was GFP mRNA, obtained from Trilink (cat. L-7601).